In conclusion the results of this study suggest

In conclusion, the results of this study suggest that daily supplementation of curcumin extracts has a positive beneficial effect against chemically induced colonic preneoplastic progression in rats induced by DMH, which provide an effective dietary chemopreventive approach to disease management. Best results were given with compound 3.
Experimental
Acknowledgment We thank and appreciate the Deanship of Scientific Research, King Saud University: This work was sponsored by a grant no. DSR-AR-2(23).
Introduction Lamivudine, chemically 4-amino-1-[(2R, 5S)-2-(hydroxyl methyl)-1, 3-oxathiolan-5-yl]-1, 2-dihydropyrimidin-2-one. Lamivudine is reverse transcriptase reported to be active against HIV-1, HIV-2 and hepatitis B virus. Lamivudine [Fig. 1] has been used for treatment of chronic hepatitis B at a lower dose than for treatment of HIV. It improves the seroconversion of e-antigen positive hepatitis B and also improves histology staging of the liver [1–3]. Tenofovir disproxil Fumarate is fumaric Tariquidar salt of the bis isopropoxy carbonyl oxy methyl ester derivative of Tenofovir [Fig. 2]. Chemically it is 9-[(R)-2- [[(isopropoxcarbonyl)-oxy] methoxy] phosphinyl] methoxy] propyl] adenine fumarate [4–7]. Dolutegravir (DTG, S/GSK-1349572, [Fig. 3] is a newly developed human immunodeficiency virus (HIV) integrase inhibitor from ViiV Healthcare (Research Triangle Park, NC, USA). DTG is an integrase strand transfer inhibitor (INSTI) that does not require ritonavir for cytochrome P450 3A4 inhibition, and preferentially blocks the strand transfer step of integration of the viral genome into the host cell\'s DNA [8], which is a two-step process mediated by the viral integrase enzyme. Like the other approved INSTIs raltegravir (RAL) and elvitegravir (EVG), DTG inhibits the binding of the integrase-viral DNA complex to host cell DNA by chelating Mg2+ ions in the active site [9]. Once integration is blocked, HIV-1 can no longer replicate, and the viral replication cycle is interrupted. In phase II trials, DTG has been shown to be highly effective at rapidly decreasing viral burden, with a concomitant increase in CD4+ cell count, in treatment-naïve patients receiving 10, 25 or 50 mg once-daily along with a nucleoside reverse transcriptase inhibitor (NRTI) background [10]. A variety of methods are in vogue for estimation of TDF, LMV and DTG individually as highlighted in the literature [11,12,13]. Literature survey reveals that Tenofovir disoproxil fumerate is estimated sensitive determination in plasma by HPLC [14], Plasma LC/MS/MS [15]and in human peripheral blood mononuclear cells methods [16], Similarly for Lamivudine estimation in human serum by HPLC [17–19], the simultaneous estimation of Lamivudine and Tenofovir disoproxil fumerate in RP- HPLC [20,21], HPTLC [22,23] and LC-MS/MS were reported.
Experimental
Results and discussion
Method validation
Conclusion
Acknowledgment