br Experimental design materials and

Experimental design, materials and methods
Acknowledgments
Data In Fig. 1 we provide deeper understanding of the recording of force distance cycles and advanced data evaluation. The process of specifity proof measurements using the example of VWF A1/A2 interaction studies INCB028050 discussed and a typical loading rate dependence plot is shown in Figs. 2 and 3. The BD simulation part ((Eqs. 1–6) and Fig. 4) gives a detailed description of the calculation of force profiles for protein chains and the fitting of rupture forces versus loading rate profiles in the MD loading-rate range can be found in Fig. 5.
Experimental design, materials and methods
Acknowledgments This study was supported by the Acentric fragment DFG within the Research group FOR1543: “Shear flow regulation of hemostasis – bridging the gap between nanomechanics and clinical presentation (SHENC)”, the FWF ProjectI 767-B11 to S.P. and P25844 to A.K., by the European Fund for Regional Development (EFRE, Regio 13) and by the Klaus Tschira Foundation (to F. G.). We thank Christian Rankl (Agilent, Linz) for his support in data evaluation, as well as Hermann Gruber (JKU, Linz) and Robert Tampé (Goethe-University Frankfurt) for providing cross linker. We acknowledge Sebastian Sturm and Jakob Thomas Bullerjahn for kindly providing the scripts for fitting the rupture force data at high loading rates and for helpful discussions.